Anti-Human DDX3X Polyclonal Antibody (HT313014)

Name: Anti-Human DDX3X Polyclonal Antibody
Brand: abinScience
SKU: HT313014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT313014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human DDX3X (Glu161-Ser410).

Target

DEAD box, X isoform, CAP-Rf, DDX3, HLP2, DEAD box protein 3, X-chromosomal, DDX3X, ATP-dependent RNA helicase DDX3X, Helicase-like protein 2, DBX

Purification

Purified by antigen affinity column.

Accession

O00571

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ATP-dependent RNA helicase DDX3X is a ~73 kDa protein. Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo-and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs. Binds RNA G-quadruplex (rG4s) structures, including those located in the 5'-UTR of NRAS mRNA. Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities (Probable).

1. Franca, R. et al. (2007) Proteins 67, 1128-37. PMID: 17357160

2. Garbelli, A. et al. (2011) PloS one 6, e19810. PMID: 21589879

3. Heaton, SM. et al. (2019) Cells 8. PMID: 31575075

4. Dolde, C. et al. (2018) Journal of cell science 131. PMID: 29222110

5. Herdy, B. et al. (2018) Nucleic acids research 46, 11592-11604. PMID: 30256975

6. Chao, CH. et al. (2006) Cancer research 66, 6579-88. PMID: 16818630

7. Botlagunta, M. et al. (2008) Oncogene 27, 3912-22. PMID: 18264132

Note

For research use only.

Images

References

Datasheet

Anti-Human DDX3X Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human DDX3X Polyclonal Antibody (HT313014)

Overview

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References

Datasheet

SDS

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