Anti-Human DDX5 Polyclonal Antibody (HA418014)

Name: Anti-Human DDX5 Polyclonal Antibody
Brand: abinScience
SKU: HA418014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA418014

Description

Anti-Human DDX5 Polyclonal Antibody (HA418014) is a rabbit polyclonal antibody detecting DDX5. Suitable for Mouse and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DDX5 (Arg68-Ser483).

Target

DDX5, DEAD box protein 5, EC:3.6.4.13, G17P1, HELR, HLR1, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P17844

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Probable ATP-dependent RNA helicase DDX5 is a ~69 kDa protein. Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-DDX5 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: 4T1 cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: Mouse brain lysate
Lane 5: Rat brain lysate

References

Datasheet

Anti-Human DDX5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human DDX5 Polyclonal Antibody (HA418014)

Overview

Images

References

Datasheet

SDS

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