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Anti-Human DGKA Polyclonal Antibody (HB083014)

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Overview
Catalog No.HB083014
Description
Anti-Human DGKA Polyclonal Antibody (HB083014) is a rabbit polyclonal antibody detecting DGKA in ELISA, IHC, WB. Suitable for Human, Bovine, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human DGKA (Met1-Thr118).
Target Diacylglycerol kinase alpha, 80 kDa diacylglycerol kinase, DAGK1, DGKA, Diglyceride kinase alpha, DAG kinase alpha, DAGK, DGK-alpha
Purification Purified by antigen affinity column.
Accession P23743
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Diacylglycerol kinase alpha (DGKA) is a ~82 kDa protein. Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro as efficiently as diacylglycerol provided it contains an arachidonoyl group. Also involved in the production of alkyl-lysophosphatidic acid, another bioactive lipid, through the phosphorylation of 1-alkyl-2-acetyl glycerol.

1. Epand, RM. et al. (2004) Biochemistry 43, 14778-83. PMID: 15544348
2. Schaap, D. et al. (1990) FEBS letters 275, 151-8. PMID: 2175712
3. Gellett, AM. et al. (2012) Biochemical and biophysical research communications 422, 758-63. PMID: 22627129
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human DGKA Polyclonal Antibody

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