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Anti-Human DGKD Polyclonal Antibody (HV696014)

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Overview
Catalog No.HV696014
Description
Anti-Human DGKD Polyclonal Antibody (HV696014) is a rabbit polyclonal antibody detecting Human DGKD. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human DGKD (Ser56-Asn314).
Target DAG kinase eta, DGK-eta, DGKH, Diacylglycerol kinase eta, Diglyceride kinase eta, EC:2.7.1.107
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q86XP1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Diacylglycerol kinase eta (DGKD) is a ~134 kDa protein. Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.

1. Murakami, T. et al. (2003) The Journal of biological chemistry 278, 34364-72. PMID: 12810723
2. Sato, M. et al. (2013) Pharmacology 92, 99-107. PMID: 23949095
3. Yasuda, S. et al. (2009) The Journal of biological chemistry 284, 29559-70. PMID: 19710016
Note For research use only
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Datasheet
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Formula
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human DGKD Polyclonal Antibody

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