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Anti-Human DHH Polyclonal Antibody (HT127014)

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Overview
Catalog No.HT127014
Description
Anti-Human DHH Polyclonal Antibody (HT127014) is a rabbit polyclonal antibody detecting DHH-N. Suitable for Human and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human DHH (Gly202-Gly396).
Target DHH, DHH-N, Desert hedgehog protein, Desert hedgehog protein N-product, EC:3.1.-.-, HHG-3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O43323
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Desert hedgehog protein (DHH) is a ~43 kDa protein. The C-terminal part of the desert hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (N-product and C-product) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-product (DHH-N). Both activities occur in the endoplasmic reticulum. Once cleaved, the C-product is degraded in the endoplasmic reticulum. Functions in cell-cell mediated juxtacrine signaling.

1. Pettigrew, CA. et al. (2014) Mechanisms of development 131, 137-49. PMID: 24342078
2. Hollier, PL. et al. (2021) Cardiovascular research 117, 2489-2501. PMID: 33063110
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human DHH Polyclonal Antibody

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