Anti-Human DNAJB11 Polyclonal Antibody (HP182014)

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Overview

Catalog No.

HP182014

Description

Anti-Human DNAJB11 Polyclonal Antibody (HP182014) is a rabbit polyclonal antibody detecting DNAJB11 in ELISA, IHC, WB. Suitable for Bovine, Dog, Human, Mouse, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Bos taurus, Dog, Human, Mouse, Pongo abelii, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DNAJB11 (Gly23-Glu333).

Target

APOBEC1-binding protein 2, DNAJB11, DnaJ homolog subfamily B member 11, ERj3p, PWP1-interacting protein 4, ER-resident protein ERdj3, ABBP-2, DnaJ protein homolog 9, EDJ, ERJ3, Human DnaJ protein 9, HDJ9, HEDJ, ER-associated DNAJ, ER-associated Hsp40 co-chaperone, ERdj3, Endoplasmic reticulum DNA J domain-containing protein 3, hDj-9

Purification

Purified by antigen affinity column.

Accession

Q9UBS4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DnaJ homolog subfamily B member 11 (DNAJB11) is a ~40 kDa protein. As a co-chaperone for HSPA5 it is required for proper folding, trafficking or degradation of proteins. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity. It is necessary for maturation and correct trafficking of PKD1.

1. Yu, M. et al. (2000) The Journal of biological chemistry 275, 24984-92. PMID: 10827079

2. Shen, Y. et al. (2005) Molecular biology of the cell 16, 40-50. PMID: 15525676

3. Cornec-Le Gall, E. et al. (2018) American journal of human genetics 102, 832-844. PMID: 29706351

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-DNAJB11 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Raji cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: A549 cell lysate
Lane 5: 293T cell lysate
Lane 6: MCF7 cell lysate
Lane 7: NIH3T3 cell lysate
Lane 8: Mouse heart lysate
Lane 9: Mouse liver lysate
Lane 10: Mouse lung lysate
Lane11: Rat heart lysate
Lane12: Rat liver lysate
Lane13: Rat lung lysate

References

Datasheet

Anti-Human DNAJB11 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-Human DNAJB11 Polyclonal Antibody (HP182014)

Overview

Images

References

Datasheet

SDS

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