Anti-Human DOT1L IP Recombinant Antibody (SAA0200) (HP484013)

Name: Anti-Human DOT1L IP Recombinant Antibody (SAA0200)
Brand: abinScience
SKU: HP484013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP484013

Description

Anti-Human DOT1L IP Recombinant Antibody (SAA0200) (HP484013) is a mouse monoclonal antibody detecting DOT1L in ELISA, IF, IP, IP-MS. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IF, IP, IP-MS

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

SAA0200

Target

DOT1-like protein, Lysine N-methyltransferase 4, DOT1L, Histone-lysine N-methyltransferase, H3 lysine-79 specific, KMT4, H3-K79-HMTase, KIAA1814, Histone H3-K79 methyltransferase

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q8TEK3

RRID

Anti-Human DOT1L IP Antibody (SAA0200) (abinScience Cat# HP484013, RRID:AB_3729734)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1L) is a ~164 kDa protein. Histone methyltransferase that methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

1. Feng, Q. et al. (2002) Current biology : CB 12, 1052-8. PMID: 12123582

2. Frisbie, VS. et al. (2024) Nature communications 15, 2467. PMID: 38503750

3. Singh, PR. et al. (2023) Nature communications 14, 8497. PMID: 38129415

4. Min, J. et al. (2003) Cell 112, 711-23. PMID: 12628190

Note

For research use only.

Isotype Control

Mouse IgG2a, kappa Recombinant Isotype Control Antibody (MOPC-173) (MF533507)

Images

SDS-PAGE

SDS-PAGE for Anti-Human DOT1L IP Antibody (SAA0200)

References

Datasheet

Anti-Human DOT1L IP Recombinant Antibody (SAA0200).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human DOT1L IP Recombinant Antibody (SAA0200) (HP484013)

Overview

Images

References

Datasheet

SDS