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Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783) (HB176013)

Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)
Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)
Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)
Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)
Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)
Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)
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Overview
Catalog No.HB176013
Description
Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783) (HB176013) is a mouse monoclonal antibody detecting DRD1/AGIA in ELISA, IF, IP, SPR, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IF, IP, SPR, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDSAA0783
Tested applications WB, IF
Target Dopamine D1 receptor, DRD1, D(1A) dopamine receptor
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P21728
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

D(1A) dopamine receptor (DRD1/AGIA) is a ~49 kDa protein. Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Forms heterotetramers with DRD3 to potentiate beta-arrestin recruitment and mediate locomotor activity.

Note For research use only.
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Images
  • Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)

    Western blot

    Western blot analysis was performed using anti-DRR1 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human DRR1 (Catalog No: HB176012)
    Lane 2: negative control

  • Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)

    SDS-PAGE

    SDS-PAGE for Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)

  • Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)

    Immunofluorescence

    Immunofluorescence staining of SHSY-5Y with Mouse monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cytoplasm.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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