Anti-Human ECHS1 Polyclonal Antibody (HB230014)

Name: Anti-Human ECHS1 Polyclonal Antibody
Brand: abinScience
SKU: HB230014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB230014

Description

Anti-Human ECHS1 Polyclonal Antibody (HB230014) is a rabbit polyclonal antibody detecting ECHS1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Pongo abelii, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ECHS1 (Ala28-Gln290).

Target

ECHS1

Purification

Purified by antigen affinity column.

Accession

P30084

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Enoyl-CoA hydratase, mitochondrial, mECH, mECH1, 4.2.1.17, 5.3.3.8, Enoyl-CoA hydratase 1, ECHS1, Short-chain enoyl-CoA hydratase, SCEH, ECHS1

Background

Enoyl-CoA hydratase, mitochondrial (ECHS1) is a ~31 kDa protein. Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond. Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16. Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate. Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation.

1. Peters, H. et al. (2014) Brain : a journal of neurology 137, 2903-8. PMID: 25125611

2. Yamada, K. et al. (2015) Journal of medical genetics 52, 691-8. PMID: 26251176

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ECHS1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Caco2 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: PC-3 cell lysate
Lane 5: 293T cell lysate
Lane 6: Mouse heart lysate
Lane 7: Rat liver lysate
Lane 8: Rat heart lysate

References

Datasheet

Anti-Human ECHS1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ECHS1 Polyclonal Antibody (HB230014)

Overview

Images

References

Datasheet

SDS

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