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Anti-Human ECI1 Polyclonal Antibody (HW666014)

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Overview
Catalog No.HW666014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ECI1 (Ala158-Gly302).
Target DCI, Enoyl-CoA delta isomerase 1, mitochondrial, 3, 2-trans-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, D3, D2-enoyl-CoA isomerase, ECI1, Delta(3), Delta(2)-enoyl-CoA isomerase
Purification Purified by antigen affinity column.
Accession P42126
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Enoyl-CoA delta isomerase 1, mitochondrial (ECI1) is a ~32 kDa protein. Key enzyme of fatty acid beta-oxidation (Probable). Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2-trans (2E) form in a range of enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs. The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length.

1. Takahashi, Y. et al. (1994) The Biochemical journal 304 ( Pt 3), 849-52. PMID: 7818490
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human ECI1 Polyclonal Antibody

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