Anti-Human EED IP Recombinant Antibody (SAA0245) (HF671013)

Name: Anti-Human EED IP Recombinant Antibody (SAA0245)
Brand: abinScience
SKU: HF671013
Price: 191 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HF671013

Species reactivity

Human

Applications

ELISA, IF, IP, IP-MS

Host species

Mouse

Isotype

IgG

Clone ID

SAA0245

Clonality

Monoclonal

Target

Polycomb protein EED, WAIT-1, EED, hEED, WD protein associating with integrin cytoplasmic tails 1, Embryonic ectoderm development protein

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O75530

RRID

Anti-Human EED IP Antibody (SAA0245) (abinScience Cat# HF671013, RRID:AB_3729779)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Polycomb protein EED is a ~50 kDa protein. Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human EED IP Antibody (SAA0245)

References

Datasheet

Anti-Human EED IP Recombinant Antibody (SAA0245).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human EED IP Recombinant Antibody (SAA0245) (HF671013)

Overview

Images

References

Datasheet

SDS

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