Anti-Human EHD1 Polyclonal Antibody (HP095014)

Name: Anti-Human EHD1 Polyclonal Antibody
Brand: abinScience
SKU: HP095014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP095014

Description

Anti-Human EHD1 Polyclonal Antibody (HP095014) is a rabbit polyclonal antibody detecting EHD1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human EHD1 (Thr20-Ser402).

Target

PAST, EHD1, PAST1, PAST homolog 1, EH domain-containing protein 1, Testilin, hPAST1

Purification

Purified by antigen affinity column.

Accession

Q9H4M9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

EH domain-containing protein 1 (EHD1) is a ~60 kDa protein. ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Plays a role in myoblast fusion.

1. Cai, B. et al. (2013) The Journal of biological chemistry 288, 30172-30180. PMID: 24019528

2. Naslavsky, N. et al. (2004) Molecular biology of the cell 15, 2410-22. PMID: 15020713

3. George, M. et al. (2007) BMC cell biology 8, 3. PMID: 17233914

4. Rahajeng, J. et al. (2010) The Journal of biological chemistry 285, 31918-22. PMID: 20801876

5. Xie, S. et al. (2019) Journal of cell science 132. PMID: 31615969

6. Lu, Q. et al. (2015) Nature cell biology 17, 228-240. PMID: 25686250

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EHD1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse lung lysate
Lane 2: Rat lung lysate

References

Datasheet

Anti-Human EHD1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human EHD1 Polyclonal Antibody (HP095014)

Overview

Images

References

Datasheet

SDS

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