Anti-Human EIF3F Polyclonal Antibody (HT342014)

Name: Anti-Human EIF3F Polyclonal Antibody
Brand: abinScience
SKU: HT342014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT342014

Description

Anti-Human EIF3F Polyclonal Antibody (HT342014) is a rabbit polyclonal antibody detecting eIF3f in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EIF3F (Gly89-Ala240).

Target

Eukaryotic translation initiation factor 3 subunit F, eIF3f, Deubiquitinating enzyme eIF3f, 3.4.19.12, Eukaryotic translation initiation factor 3 subunit 5, eIF-3-epsilon, eIF3 p47, EIF3F, EIF3S5

Purification

Purified by antigen affinity column.

Accession

O00303

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Eukaryotic translation initiation factor 3 subunit F (EIF3F) is a ~37 kDa protein. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

1. Masutani, M. et al. (2007) The EMBO journal 26, 3373-83. PMID: 17581632

2. Lee, AS. et al. (2015) Nature 522, 111-4. PMID: 25849773

3. Lee, AS. et al. (2016) Nature 536, 96-9. PMID: 27462815

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EIF3F polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: PC-12 cell lysate
Lane 4: HepG2 cell lysate

References

Datasheet

Anti-Human EIF3F Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human EIF3F Polyclonal Antibody (HT342014)

Overview

Images

References

Datasheet

SDS

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