Anti-Human EIF4A1 Polyclonal Antibody (HX179014)

Name: Anti-Human EIF4A1 Polyclonal Antibody
Brand: abinScience
SKU: HX179014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX179014

Description

Anti-Human EIF4A1 Polyclonal Antibody (HX179014) is a rabbit polyclonal antibody detecting EIF4A1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human EIF4A1 (Thr242-Ile406).

Target

Eukaryotic initiation factor 4A-I, eIF4A-I, eIF-4A-I, EIF4A, EIF4A1, DDX2A, ATP-dependent RNA helicase eIF4A-1,

Purification

Purified by antigen affinity column.

Accession

P60842

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Eukaryotic initiation factor 4A-I (EIF4A1) is a ~46 kDa protein. ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth.

1. Wolf, A. et al. (2010) The Journal of cell biology 188, 463-71. PMID: 20156963

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-EIF4A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC12 cell lysate
Lane 3: A431 cell lysate