Anti-Human ELAVL1 Polyclonal Antibody (HS864014)

Name: Anti-Human ELAVL1 Polyclonal Antibody
Brand: abinScience
SKU: HS864014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HS864014

Description

Anti-Human ELAVL1 Polyclonal Antibody (HS864014) is a rabbit polyclonal antibody detecting ELAVL1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Xenopus laevis, and Xenopus tropicalis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ELAVL1 (Gly18-Gln198).

Target

ELAV-like protein 1, HuR, ELAVL1, Hu-antigen R, HUR

Purification

Purified by antigen affinity column.

Accession

Q15717

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ELAV-like protein 1 (ELAVL1) is a ~36 kDa protein. RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation. Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs.

1. Tran, H. et al. (2003) Molecular and cellular biology 23, 7177-88. PMID: 14517288

2. Doller, A. et al. (2008) Molecular and cellular biology 28, 2608-25. PMID: 18285462

3. Chen, X. et al. (2019) Nature cell biology 21, 978-990. PMID: 31358969

4. Zhu, S. et al. (2020) Nature communications 11, 1685. PMID: 32245947

5. Tran, H. et al. (2004) Molecular cell 13, 101-11. PMID: 14731398

6. Meisner, NC. et al. (2007) Nature chemical biology 3, 508-15. PMID: 17632515

7. Wang, H. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 373-80. PMID: 23519412

8. Ma, WJ. et al. (1996) The Journal of biological chemistry 271, 8144-51. PMID: 8626503

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ELAVL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: A549 cell lysate
Lane 3: K562 cell lysate
Lane 4: H1299 cell lysate
Western blot

Western blot analysis was performed using anti-ELAVL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A431 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Lane 4: A549 cell lysate
Lane 5: N2A cell lysate
Lane 6: K562 cell lysate
Lane 7: H1299 cell lysate

References

Datasheet

Anti-Human ELAVL1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human ELAVL1 Polyclonal Antibody (HS864014)

Overview

Images

References

Datasheet

SDS

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