Anti-Human EMC2 Polyclonal Antibody (HC225014)

Name: Anti-Human EMC2 Polyclonal Antibody
Brand: abinScience
SKU: HC225014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HC225014

Description

Anti-Human EMC2 Polyclonal Antibody (HC225014) is a rabbit polyclonal antibody detecting EMC2. Suitable for Rat, Mouse, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EMC2 (Met1-Ser297).

Target

EMC2, ER membrane protein complex subunit 2, KIAA0103, TPR repeat protein 35, TTC35, Tetratricopeptide repeat protein 35

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q15006

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

ER membrane protein complex subunit 2 (EMC2) is a ~34 kDa protein. Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors.

1. Guna, A. et al. (2018) Science (New York, N.Y.) 359, 470-473. PMID: 29242231

2. Shurtleff, MJ. et al. (2018) eLife 7. PMID: 29809151

3. Chitwood, PJ. et al. (2018) Cell 175, 1507-1519.e16. PMID: 30415835

4. Pleiner, T. et al. (2020) Science (New York, N.Y.) 369, 433-436. PMID: 32439656

5. O'Donnell, JP. et al. (2020) eLife 9. PMID: 32459176

6. Pleiner, T. et al. (2021) Molecular cell 81, 2693-2704.e12. PMID: 33964204

Note

For research use only

Images

References

Datasheet

Anti-Human EMC2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human EMC2 Polyclonal Antibody (HC225014)

Overview

Images

References

Datasheet

SDS

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