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Anti-Human EPHA2 Antibody (SAA0832) (HB738107)

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Anti-Human EPHA2 Antibody (SAA0832)

Anti-Human EPHA2 Antibody (SAA0832)

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Datasheet

Overview

Catalog No.	HB738107
Description	Anti-Human EPHA2 Antibody (SAA0832) [SAA0832] (HB738107) is a mouse monoclonal antibody detecting EPHA2 in ELISA, FCM, FuncS, IF, WB. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM, FuncS, IF, WB
Host species	Mouse
Isotype	IgG2a
Clone ID	SAA0832
Clonality	Monoclonal
Target	Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, EPHA2, Ephrin type-A receptor 2, ECK
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P29317
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Ephrin type-A receptor 2 (EPHA2) is a ~108 kDa protein. Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration.
Note	For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human EPHA2 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EPHA2 antibody monoclonal antibody (Catalog # HB738107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human EPHA2 Antibody (SAA0832).pdf

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Anti-Human EPHA2 Antibody (SAA0832)