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Anti-Human ERO1B Polyclonal Antibody (HC233014)

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Overview
Catalog No.HC233014
Description
Anti-Human ERO1B Polyclonal Antibody (HC233014) is a rabbit polyclonal antibody detecting ERO1B. Suitable for Human and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ERO1B (Thr36-Arg467).
Target EC:1.8.4.-, ERO1-L-beta, ERO1-like protein beta, ERO1B, ERO1LB, Endoplasmic reticulum oxidoreductase beta, Endoplasmic reticulum oxidoreductin-1-like protein B, Oxidoreductin-1-L-beta
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q86YB8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

ERO1-like protein beta (ERO1B) is a ~53 kDa protein. Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2 (50% of P4HB reoxidation rate), as well as PDIA3, PDIA4, PDIA6 and NXNDC12 (<10%). Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. May be involved in oxidative proinsulin folding in pancreatic cells, hence may play a role in glucose homeostasis.

Note For research use only
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Formula
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C₂ (Working Conc.)
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Anti-Human ERO1B Polyclonal Antibody

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