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Anti-Human ESRP1 Polyclonal Antibody (HP161014)

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Overview
Catalog No.HP161014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ESRP1 (Met1-Ser193).
Target RNA-binding protein 35A, Epithelial splicing regulatory protein 1, ESRP1, RNA-binding motif protein 35A, RBM35A
Purification Purified by antigen affinity column.
Accession Q6NXG1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Epithelial splicing regulatory protein 1 (ESRP1) is a ~75 kDa protein. mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

1. Warzecha, CC. et al. (2009) Molecular cell 33, 591-601. PMID: 19285943
Note For research use only.
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