Anti-Human ESRRB Polyclonal Antibody (HF928014)

Name: Anti-Human ESRRB Polyclonal Antibody
Brand: abinScience
SKU: HF928014
Price: 96 USD
Availability: InStock

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Overview
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SDS

Overview

Catalog No.

HF928014

Description

Anti-Human ESRRB Polyclonal Antibody (HF928014) is a rabbit polyclonal antibody detecting ESRRB in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ESRRB (Gly235-Val433).

Target

ERR-beta, NR3B2, Estrogen-related receptor beta, ESRL2, Estrogen receptor-like 2, ERR beta-2, ESRRB, Nuclear receptor subfamily 3 group B member 2, Steroid hormone receptor ERR2, ERRB2

Purification

Purified by antigen affinity column.

Accession

O95718

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Steroid hormone receptor ERR2 (ESRRB) is a ~48 kDa protein. Transcription factor that binds a canonical ESRRB recognition (ERRE) sequence 5'TCAAGGTCA-3' localized on promoter and enhancer of targets genes regulating their expression or their transcription activity. Plays a role, in a LIF-independent manner, in maintainance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF signaling pathway and Wnt signaling pathways. Involved in morula development (2-16 cells embryos) by acting as a regulator at the 8-cell stage. Upon FGF signaling pathway activation, interacts with KDM1A by directly binding to enhancer site of ELF5 and EOMES and activating their transcription leading to self-renewal of trophoblast stem cells. Also regulates expression of multiple rod-specific genes and is required for survival of this cell type.

1. Zhou, W. et al. (2007) Molecular and cellular endocrinology 278, 52-62. PMID: 17920186

2. Bombail, V. et al. (2010) Molecular and cellular endocrinology 314, 53-61. PMID: 19755138

3. Cho, Y. et al. (2013) The Journal of biological chemistry 288, 25207-25218. PMID: 23836911

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ESRRB polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse heart lysate
Lane 2: Rat heart lysate

References

Datasheet

Anti-Human ESRRB Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ESRRB Polyclonal Antibody (HF928014)

Overview

Images

References

Datasheet

SDS

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