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Anti-Human FCN2 Polyclonal Antibody (HS794014)

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Overview
Catalog No.HS794014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human FCN2 (Thr47-Asn300).
Target Hucolin, FCNL, Collagen/fibrinogen domain-containing protein 2, 37 kDa elastin-binding protein, Serum lectin p35, Ficolin-2, EBP-37, FCN2, Ficolin-beta, L-ficolin, Ficolin-B
Purification Purified by antigen affinity column.
Accession Q15485
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ficolin-2 (FCN2) is a ~34 kDa protein. Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Specifically recognizes and binds carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway. Specifically binds N-Acetylglucosamine (GlcNAc), as well as phosphocholine and lipoteichoic acid moieties on the surface of pathogens. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect.

1. Matsushita, M. et al. (2000) Journal of immunology (Baltimore, Md. : 1950) 164, 2281-4. PMID: 10679061
2. Lynch, NJ. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 172, 1198-202. PMID: 14707097
3. Kuraya, M. et al. (2005) Immunobiology 209, 689-97. PMID: 15804047
4. Garlatti, V. et al. (2007) The EMBO journal 26, 623-33. PMID: 17215869
5. Vassal-Stermann, E. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 5699-708. PMID: 25344472
6. Matsushita, M. et al. (1996) The Journal of biological chemistry 271, 2448-54. PMID: 8576206
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human FCN2 Polyclonal Antibody

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