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Anti-Human FIG4 Polyclonal Antibody (HP232014)

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Overview
Catalog No.HP232014
Description
Anti-Human FIG4 Polyclonal Antibody (HP232014) is a rabbit polyclonal antibody detecting FIG4. Suitable for Human and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human FIG4 (Gly216-His665).
Target EC:3.1.3.-, EC:3.1.3.16, EC:3.1.3.36, EC:3.1.3.86, FIG4, KIAA0274, Phosphatidylinositol 3,5-bisphosphate 5-phosphatase, Polyphosphoinositide phosphatase, SAC domain-containing protein 3, SAC3, Serine-protein phosphatase FIG4
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q92562
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Polyphosphoinositide phosphatase (FIG4) is a ~103 kDa protein. Dual specificity phosphatase component of the PI(3,5)P2 regulatory complex which regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the dephosphorylation of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) to form phosphatidylinositol 3-phosphate. Has serine-protein phosphatase activity acting on PIKfyve to stimulate its lipid kinase activity, its catalytically activity being required for maximal PI(3,5)P2 production. In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide and although displaying preferences for PtdIns(3,5)P2, it is capable of hydrolyzing PtdIns(3,4,5)P3 and PtdIns(4,5)P2, at least in vitro.

1. Sbrissa, D. et al. (2007) The Journal of biological chemistry 282, 23878-91. PMID: 17556371
2. Lees, JA. et al. (2020) Molecular cell 80, 736-743.e4. PMID: 33098764
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human FIG4 Polyclonal Antibody

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