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Anti-Human FSTL1 Polyclonal Antibody (HC289014)

Anti-Human FSTL1 Polyclonal Antibody
Anti-Human FSTL1 Polyclonal Antibody
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Overview
Catalog No.HC289014
Description
Anti-Human FSTL1 Polyclonal Antibody (HC289014) is a rabbit polyclonal antibody detecting FSTL1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human FSTL1 (Ser116-Thr266).
Target FSTL1, Follistatin-related protein 1, Follistatin-like protein 1, FRP,
Purification Purified by antigen affinity column.
Accession Q12841
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Follistatin-related protein 1 (FSTL1) is a ~34 kDa protein. Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation. Plays a role in the development of the central nervous system, skeletal system, lungs, and ureter. Promotes endothelial cell survival, migration and differentiation into network structures in an AKT-dependent manner. Also promotes survival of cardiac myocytes. Initiates various signaling cascades by activating different receptors on the cell surface such as DIP2A, TLR4 or BMP receptors.

1. Murakami, K. et al. (2012) FEBS letters 586, 319-24. PMID: 22265692
2. Jin, X. et al. (2017) Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 44, 1616-1628. PMID: 29212066
3. Ouchi, N. et al. (2010) The Journal of biological chemistry 285, 7127-34. PMID: 20054002
Note For research use only.
Images
  • Anti-Human FSTL1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-FSTL1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: MCF-7 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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