Anti-Human G6PD Polyclonal Antibody (HY124014)

Name: Anti-Human G6PD Polyclonal Antibody
Brand: abinScience
SKU: HY124014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY124014

Description

Anti-Human G6PD Polyclonal Antibody (HY124014) is a rabbit polyclonal antibody detecting G6PD in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human G6PD (Met1-Leu515).

Target

Glucose-6-phosphate 1-dehydrogenase, G6PD

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P11413

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glucose-6-phosphate 1-dehydrogenase (G6PD) is a ~59 kDa protein. Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. Also catalyzes the conversion of NAADPH, which is produced by enzymes such as DUOX1, DUOX2 and NOX5 from NAADP and promotes Ca(2+) signaling during T cell activation, back to NAADP.

1. Gu, F. et al. (2021) Science signaling 14, eabe3800. PMID: 34784249

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-G6PD polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: LO2 cell lysate
Lane 3: A431 cell lysate
Lane 4: A549 cell lysate
Western blot

Western blot analysis was performed using anti-G6PD polyclonal antibody at 1ug/mL on mouse hippocampus.

References

Datasheet

Anti-Human G6PD Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human G6PD Polyclonal Antibody (HY124014)

Overview

Images

References

Datasheet

SDS

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