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Anti-Human GADD45GIP1 Polyclonal Antibody (HP426014)

Anti-Human GADD45GIP1 Polyclonal Antibody
Anti-Human GADD45GIP1 Polyclonal Antibody
Anti-Human GADD45GIP1 Polyclonal Antibody
Anti-Human GADD45GIP1 Polyclonal Antibody
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Overview
Catalog No.HP426014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GADD45GIP1 (Thr47-Ser222).
Target CRIF1, GADD45GIP1, 39S ribosomal protein L59, mitochondrial, PLINP1, PLINP, MRP-L59, CKII beta-associating protein, Papillomavirus L2-interacting nuclear protein 1, PLINP-1, PRG6, CR6-interacting factor 1, MRPL59, p53-responsive gene 6 protein, Growth arrest and DNA damage-inducible proteins-interacting protein 1, Mitochondrial large ribosomal subunit protein mL64
Purification Purified by antigen affinity column.
Accession Q8TAE8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Large ribosomal subunit protein mL64 (GADD45GIP1) is a ~25 kDa protein. Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but also occur in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.

Note For research use only.
Images
  • Anti-Human GADD45GIP1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Human GADD45GIP1 polyclonal antibody at 1ug/mL on recombinant Human GADD45GIP1 (Catalog No. HP426012).

  • Anti-Human GADD45GIP1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-GADD45GIP1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: SKOV3 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: U251 cell lysate
    Lane 4: Hela cell lysate
    Lane 5: 293T cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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