Anti-Human GAPDH Polyclonal Antibody (HY030014)

Name: Anti-Human GAPDH Polyclonal Antibody
Brand: abinScience
SKU: HY030014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY030014

Description

Anti-Human GAPDH Polyclonal Antibody (HY030014) is a rabbit polyclonal antibody detecting GAPDH in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Cat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GAPDH (Met1-Glu335).

Target

GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P04406, P16858, P04797

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ~36 kDa protein. Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.

1. Tisdale, EJ. (2002) The Journal of biological chemistry 277, 3334-41. PMID: 11724794

2. Ercolani, L. et al. (1988) The Journal of biological chemistry 263, 15335-41. PMID: 3170585

3. Arif, A. et al. (2012) Molecular and cellular biology 32, 5046-55. PMID: 23071094

4. Gao, X. et al. (2013) Cell host & microbe 13, 87-99. PMID: 23332158

5. Gao, X. et al. (2016) The Journal of biological chemistry 291, 18232-8. PMID: 27387501

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GAPDH polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: K562 cell lysate
Lane 5: PC-3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: HT-29 cell lysate

References

Datasheet

Anti-Human GAPDH Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GAPDH Polyclonal Antibody (HY030014)

Overview

Images

References

Datasheet

SDS

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