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Anti-Human GATA1 Polyclonal Antibody (HB017014)

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Overview
Catalog No.HB017014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GATA1 (Ala211-Asp300).
Target GATA-1, GF-1, NF-E1 DNA-binding protein, ERYF1, GF1, Erythroid transcription factor, GATA-binding factor 1, GATA1, Eryf1
Purification Purified by antigen affinity column.
Accession P15976
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Erythroid transcription factor (GATA1) is a ~42 kDa protein. Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.

1. Ludwig, LS. et al. (2022) Blood 139, 2534-2546. PMID: 35030251
2. Mizuta, S. et al. (2014) Genes to cells : devoted to molecular & cellular mechanisms 19, 28-51. PMID: 24245781
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human GATA1 Polyclonal Antibody

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