Anti-Human GBP1 Polyclonal Antibody (HW466014)

Name: Anti-Human GBP1 Polyclonal Antibody
Brand: abinScience
SKU: HW466014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HW466014

Description

Anti-Human GBP1 Polyclonal Antibody (HW466014) is a rabbit polyclonal antibody detecting GBP1 in ELISA, IHC, WB. Suitable for Human and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human GBP1 (Met1-Ser592).

Target

Guanine nucleotide-binding protein 1, HuGBP-1, GBP-1, Interferon-induced guanylate-binding protein 1, GBP1, GTP-binding protein 1, Guanylate-binding protein 1

Purification

Purified by antigen affinity column.

Accession

P32455

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Guanylate-binding protein 1 (GBP1) is a ~67 kDa protein. Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. Hydrolyzes GTP to GMP in two consecutive cleavage reactions: GTP is first hydrolyzed to GDP and then to GMP in a processive manner. Following infection, recruited to the pathogen-containing vacuoles or vacuole-escaped bacteria and promotes both inflammasome assembly and autophagy. Acts as a positive regulator of inflammasome assembly by facilitating the detection of inflammasome ligands from pathogens. Involved in the lysis of pathogen-containing vacuoles, releasing pathogens into the cytosol.

1. Ghosh, A. et al. (2006) Nature 440, 101-4. PMID: 16511497

2. Nordmann, A. et al. (2012) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26, 1290-300. PMID: 22106366

3. Li, P. et al. (2017) Nature 551, 378-383. PMID: 29144452

4. Fisch, D. et al. (2019) The EMBO journal 38, e100926. PMID: 31268602

5. Kutsch, M. et al. (2020) The EMBO journal 39, e104926. PMID: 32510692

6. Santos, JC. et al. (2020) Nature communications 11, 3276. PMID: 32581219

7. Fisch, D. et al. (2023) Science (New York, N.Y.) 382, eadg2253. PMID: 37797010

8. Schwemmle, M. et al. (1994) The Journal of biological chemistry 269, 11299-305. PMID: 7512561

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GBP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: MDA-MB-231 cell lysate
Lane 2: HUVEC cell lysate
Lane 3: Hela cell lysate
Lane 4: Jurkat cell lysate

References

Datasheet

Anti-Human GBP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GBP1 Polyclonal Antibody (HW466014)

Overview

Images

References

Datasheet

SDS

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