Anti-Human GCK Polyclonal Antibody (HW504014)

Name: Anti-Human GCK Polyclonal Antibody
Brand: abinScience
SKU: HW504014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HW504014

Description

Anti-Human GCK Polyclonal Antibody (HW504014) is a rabbit polyclonal antibody detecting GCK in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human GCK (Met1-Gln465).

Target

Hexokinase-4, Hexokinase type IV, HK4, HK IV, Glucokinase, Hexokinase-D, GCK

Purification

Purified by antigen affinity column.

Accession

P35557

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Hexokinase-4 (GCK) is a ~52 kDa protein. Catalyzes the phosphorylation of hexose, such as D-glucose, D-fructose and D-mannose, to hexose 6-phosphate (D-glucose 6-phosphate, D-fructose 6-phosphate and D-mannose 6-phosphate, respectively). Compared to other hexokinases, has a weak affinity for D-glucose, and is effective only when glucose is abundant. Mainly expressed in pancreatic beta cells and the liver and constitutes a rate-limiting step in glucose metabolism in these tissues. Since insulin secretion parallels glucose metabolism and the low glucose affinity of GCK ensures that it can change its enzymatic activity within the physiological range of glucose concentrations, GCK acts as a glucose sensor in the pancreatic beta cell. In pancreas, plays an important role in modulating insulin secretion.

1. Christesen, HB. et al. (2002) Diabetes 51, 1240-6. PMID: 11916951

2. Cuesta-Muñoz, AL. et al. (2004) Diabetes 53, 2164-8. PMID: 15277402

3. Heredia, VV. et al. (2006) The Journal of biological chemistry 281, 40201-7. PMID: 17082186

4. Estalella, I. et al. (2008) Journal of human genetics 53, 460-466. PMID: 18322640

5. Pal, P. et al. (2009) Biochemistry 48, 814-6. PMID: 19146401

6. Raimondo, A. et al. (2014) Human molecular genetics 23, 6432-40. PMID: 25015100

7. Xu, LZ. et al. (1995) Biochemistry 34, 6083-92. PMID: 7742312

8. Takeda, J. et al. (1993) The Journal of biological chemistry 268, 15200-4. PMID: 8325892

Note

For research use only.

Images

References

Datasheet

Anti-Human GCK Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human GCK Polyclonal Antibody (HW504014)

Overview

Images

References

Datasheet

SDS

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