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Anti-Human GDF7 Polyclonal Antibody (HV295014)

Anti-Human GDF7 Polyclonal Antibody
Anti-Human GDF7 Polyclonal Antibody
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Overview
Catalog No.HV295014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GDF7 (Thr322-Arg450).
Target Growth/differentiation factor 7, GDF-7, GDF7
Purification Purified by antigen affinity column.
Accession Q7Z4P5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Growth/differentiation factor 7 (GDF7) is a ~46 kDa protein. May play an active role in the motor area of the primate neocortex.

Note For research use only.
Images
  • Anti-Human GDF7 Polyclonal Antibody

    Immunofluorescence

    U2OS cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting GDF7 (HV295014) at a concentration of 20.75 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of GDF7 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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