Anti-Human GEN1 Polyclonal Antibody (HD546014)

Name: Anti-Human GEN1 Polyclonal Antibody
Brand: abinScience
SKU: HD546014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HD546014

Description

Anti-Human GEN1 Polyclonal Antibody (HD546014) is a rabbit polyclonal antibody detecting GEN1. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GEN1 (Met1-Pro473).

Target

EC:3.1.-.-, Flap endonuclease GEN homolog 1, GEN1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q17RS7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Flap endonuclease GEN homolog 1 (GEN1) is a ~102 kDa protein. Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction.

1. Ip, SC. et al. (2008) Nature 456, 357-61. PMID: 19020614

2. Lee, SH. et al. (2015) eLife 4. PMID: 26682650

3. Chan, YW. et al. (2015) Nucleic acids research 43, 10882-92. PMID: 26578604

4. Shah Punatar, R. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 443-450. PMID: 28049850

Note

For research use only

Images

References

Datasheet

Anti-Human GEN1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GEN1 Polyclonal Antibody (HD546014)

Overview

Images

References

Datasheet

SDS

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