Anti-Human GFPT1 Polyclonal Antibody (HC425014)

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Overview
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Datasheet

Overview

Catalog No.

HC425014

Description

Anti-Human GFPT1 Polyclonal Antibody (HC425014) is a rabbit polyclonal antibody detecting GFPT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GFPT1 (Ser341-Gly683).

Target

Glutamine:fructose-6-phosphate amidotransferase 1, GFAT 1, Hexosephosphate aminotransferase 1, D-fructose-6-phosphate amidotransferase 1, GFPT1, GFPT, GFAT, Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1, GFAT1

Purification

Purified by antigen affinity column.

Accession

Q06210

RRID

Anti-Human GFPT1 Polyclonal Antibody (abinScience Cat# HC425014, RRID:AB_3730562)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (GFPT1) is a ~78 kDa protein. Rate-limiting enzyme of the hexosamine biosynthetic pathway (HBP) that catalyzes the formation of glucosamine-6-phosphate from fructose-6-phosphate and glutamine, thereby controlling the flux of glucose into this pathway. Inhibited by UDP-N-acetylglucosamine (UDP-GlcNAc) through a feedback loop. Fine-tunes the metabolic fluctuations of UDP-GlcNAc and its impacts on hyaluronan synthesis during tissue remodeling. Via control of the HPB, regulates the availability of precursors for N- and O-linked protein glycosylation and modulates peripheral clock oscillation.

1. Ruegenberg, S. et al. (2020) Nature communications 11, 687. PMID: 32019926

2. Kroef, V. et al. (2022) eLife 11. PMID: 35229715

3. Oikari, S. et al. (2016) Glycobiology 26, 710-22. PMID: 26887390

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GFPT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate

References

Datasheet

Anti-Human GFPT1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GFPT1 Polyclonal Antibody (HC425014)

Overview

Images

References

Datasheet

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