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Anti-Human GFRA2 Polyclonal Antibody (HT422014)

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Overview
Catalog No.HT422014
Description
Anti-Human GFRA2 Polyclonal Antibody (HT422014) is a rabbit polyclonal antibody detecting GFRA2 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GFRA2 (Asp226-Ser444).
Target TGF-beta-related neurotrophic factor receptor 2, RETL2, GDNFR-alpha-2, GFRA2, GFR-alpha-2, Neurturin receptor alpha, TRNR2, GDNF receptor beta, GDNF family receptor alpha-2, NRTNR-alpha, RET ligand 2, NTNR-alpha, GDNFRB, GDNF receptor alpha-2, GDNFR-beta
Purification Purified by antigen affinity column.
Accession O00451
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

GDNF family receptor alpha-2 (GFRA2) is a ~51 kDa protein. Receptor for neurturin (NRTN), a growth factor that supports the survival of sympathetic neurons. NRTN-binding leads to autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor.

1. Cik, M. et al. (2000) The Journal of biological chemistry 275, 27505-12. PMID: 10829012
2. Sandmark, J. et al. (2018) The Journal of biological chemistry 293, 5492-5508. PMID: 29414779
3. Li, J. et al. (2019) eLife 8. PMID: 31535977
4. Baloh, RH. et al. (1997) Neuron 18, 793-802. PMID: 9182803
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human GFRA2 Polyclonal Antibody

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