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Anti-Human GINS2 Polyclonal Antibody (HP979014)

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Overview
Catalog No.HP979014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human GINS2 (Met1-Phe185).
Target GINS complex subunit 2, PSF2, DNA replication complex GINS protein PSF2, GINS2
Purification Purified by antigen affinity column.
Accession Q9Y248
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

DNA replication complex GINS protein PSF2 (GINS2) is a ~21 kDa protein. Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.

1. Kamada, K. et al. (2007) Nature structural & molecular biology 14, 388-96. PMID: 17417653
2. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425
3. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004
4. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328
5. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human GINS2 Polyclonal Antibody

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