Anti-Human Glutathione hydrolase Polyclonal Antibody (HB811014)

Name: Anti-Human Glutathione hydrolase Polyclonal Antibody
Brand: abinScience
SKU: HB811014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HB811014

Description

Anti-Human Glutathione hydrolase Polyclonal Antibody (HB811014) is a rabbit polyclonal antibody detecting Glutathione hydrolase 1 proenzyme in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human GGT1 (Ser122-Ser299).

Target

CD224, EC:2.3.2.2, EC:3.4.19.13, EC:3.4.19.14, GGT, GGT 1, GGT1, Gamma-glutamyltransferase 1, Gamma-glutamyltranspeptidase 1, Glutathione hydrolase 1 heavy chain, Glutathione hydrolase 1 light chain, Glutathione hydrolase 1 proenzyme, Leukotriene-C4 hydrolase

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P19440, P36268, A6NGU5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glutathione hydrolase 1 proenzyme is a ~61 kDa protein. Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates (such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate, MCTR1) and other gamma-glutamyl compounds (such as leukotriene C4, LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.

1. Castonguay, R. et al. (2007) Biochemistry 46, 12253-62. PMID: 17924658

2. Wickham, S. et al. (2011) Analytical biochemistry 414, 208-14. PMID: 21447318

3. Dalli, J. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, 12232-12237. PMID: 27791009

4. Ikeda, Y. et al. (1995) The Journal of biological chemistry 270, 22223-8. PMID: 7673200

5. Ikeda, Y. et al. (1995) The Journal of biological chemistry 270, 12471-5. PMID: 7759490

6. Ikeda, Y. et al. (1993) The Journal of biological chemistry 268, 3980-5. PMID: 8095045

7. Ikeda, Y. et al. (1996) Journal of biochemistry 119, 1166-70. PMID: 8827453

Note

For research use only

Images

References

Datasheet

Anti-Human Glutathione hydrolase Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human Glutathione hydrolase Polyclonal Antibody (HB811014)

Overview

Images

References

Datasheet

SDS