Anti-Human GPC1 Recombinant Antibody (SAA0530) (HW614107)

Name: Anti-Human GPC1 Recombinant Antibody (SAA0530)
Brand: abinScience
SKU: HW614107
Price: 163 USD
Availability: InStock

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Overview

Catalog No.	HW614107
Species reactivity	Human
Applications	ELISA, FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	SAA0530
Clonality	Monoclonal
Target	Glypican-1, GPC1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P35052
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.09% Sodium azide. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Glypican-1 (GPC1) is a ~61 kDa protein. Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human GPC1 antibody.A431 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human GPC1 antibody monoclonal antibody (Catalog # HW614107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Overview