Anti-Human GPC3 Recombinant Nanobody (HN3) (HW310033)

Name: Anti-Human GPC3 Recombinant Nanobody (HN3)
Brand: abinScience
SKU: HW310033
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW310033

Description

Anti-Human GPC3 Nanobody (HN3) [HN3] (HW310033) is a alpaca monoclonal antibody detecting GPC3 in ELISA, Neutralization. Suitable for Human.

Species reactivity

Human

Applications

ELISA, Neutralization

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

HN3

Clonality

Monoclonal

Target

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P51654

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063

2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623

3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449

4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790

Note

For research use only.

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