Anti-Human Glypican 3 Polyclonal Antibody (HW310014)

Name: Anti-Human Glypican 3 Polyclonal Antibody
Brand: abinScience
SKU: HW310014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW310014

Description

Anti-Human GPC3 Polyclonal Antibody (HW310014) is a rabbit polyclonal antibody detecting GPC3. Suitable for Mouse, Human, and Rat.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GPC3 (Leu205-Gly322).

Target

GPC3, GTR2-2, Glypican-3, Glypican-3 alpha subunit, Glypican-3 beta subunit, Intestinal protein OCI-5, MXR7, OCI5

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P51654

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Glypican-3 (GPC3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063

2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623

3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449

4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790

Note

For research use only

Images

References

Datasheet