Anti-Human GPX4 Recombinant Antibody (SAA0487) (HW551013)

Name: Anti-Human GPX4 Recombinant Antibody (SAA0487)
Brand: abinScience
SKU: HW551013
Price: 191 USD
Availability: InStock

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100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW551013

Description

Anti-Human GPX4 Recombinant Antibody (SAA0487) (HW551013) is a mouse monoclonal antibody detecting GPX4 in DB, ELISA, IHC, IP, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

DB, ELISA, IHC, IP, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

SAA0487

Target

PHGPx, Glutathione peroxidase 4, GPX4, GSHPx-4, GPx-4, Phospholipid hydroperoxide glutathione peroxidase

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P36969

RRID

Anti-Human GPX4/PHGPx Antibody (SAA0487) (abinScience Cat# HW551013, RRID:AB_3726011)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Phospholipid hydroperoxide glutathione peroxidase GPX4 (PHGPx) is a ~22 kDa protein. Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins. Can also reduce cholesterol hydroperoxide and thymine hydroperoxide. Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation. Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species. The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis.

1. Zheng, P. et al. (2025) Nature chemical biology 21, 1420-1432. PMID: 40281343

2. Yang, WS. et al. (2014) Cell 156, 317-331. PMID: 24439385

3. Sutherland, M. et al. (2001) The Biochemical journal 353, 91-100. PMID: 11115402

4. Schwarz, M. et al. (2023) Redox biology 59, 102593. PMID: 36608588

5. Scheerer, P. et al. (2007) Biochemistry 46, 9041-9. PMID: 17630701

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human GPX4/PHGPx Antibody (SAA0487)

References

Datasheet

Anti-Human GPX4 Recombinant Antibody (SAA0487).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human GPX4 Recombinant Antibody (SAA0487) (HW551013)

Overview

Images

References

Datasheet

SDS