Anti-Human GSDMD Polyclonal Antibody (HX874014)

Name: Anti-Human GSDMD Polyclonal Antibody
Brand: abinScience
SKU: HX874014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX874014

Description

Anti-Human GSDMD Polyclonal Antibody (HX874014) is a rabbit polyclonal antibody detecting GSDMD in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GSDMD (Pro278-His484).

Target

GSDMD-CT, hGSDMD-NTD, Gasdermin domain-containing protein 1, Gasdermin-D, DFNA5L, GSDMD-NT, hGSDMD-CTD, GSDMDC1, GSDMD

Purification

Purified by antigen affinity column.

Accession

P57764

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Gasdermin-D (GSDMD) is a ~52 kDa protein. Precursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals. This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-D, N-terminal) binds to membranes and forms pores, triggering pyroptosis.

1. Shi, J. et al. (2015) Nature 526, 660-5. PMID: 26375003

2. Kayagaki, N. et al. (2015) Nature 526, 666-71. PMID: 26375259

3. Ding, J. et al. (2016) Nature 535, 111-6. PMID: 27281216

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-GSDMD polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: THP-1 cell lysate