Anti-Human GSPT1 Polyclonal Antibody (HB747014)

Name: Anti-Human GSPT1 Polyclonal Antibody
Brand: abinScience
SKU: HB747014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HB747014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GSPT1 (Lys71-Asp499).

Target

Eukaryotic peptide chain release factor subunit 3a, GSPT1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, G1 to S phase transition protein 1 homolog, eRF3a, ERF3A

Purification

Purified by antigen affinity column.

Accession

P15170

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1) is a ~55 kDa protein. GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA. GSPT1/ERF3A mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. GTP hydrolysis by GSPT1/ERF3A induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes.

1. Chauvin, C. et al. (2005) Molecular and cellular biology 25, 5801-11. PMID: 15987998

2. Cheng, Z. et al. (2009) Genes & development 23, 1106-18. PMID: 19417105

3. Hoshino, S. et al. (1989) The EMBO journal 8, 3807-14. PMID: 2511002

4. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242

5. Alkalaeva, EZ. et al. (2006) Cell 125, 1125-36. PMID: 16777602

6. Feng, T. et al. (2014) Molecular cell 53, 645-54. PMID: 24486019

7. Wang, X. et al. (2019) Cell 176, 625-635.e14. PMID: 30682371

Note

For research use only.

Images

References

Datasheet

Anti-Human GSPT1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human GSPT1 Polyclonal Antibody (HB747014)

Overview

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Datasheet

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