Anti-Human H4C1 Polyclonal Antibody (HX879014)

Name: Anti-Human H4C1 Polyclonal Antibody
Brand: abinScience
SKU: HX879014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX879014

Description

Anti-Human H4C1 Polyclonal Antibody (HX879014) is a rabbit polyclonal antibody detecting H4C1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Acropora formosa, and Agaricus bisporus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human H4C1 (Asn26-Gly103).

Target

HIST1H4D, HIST2H4A, H4FE, H4FO, H4/D, H4/A, HIST1H4E, HIST1H4F, H4FG, HIST1H4L, H4FD, H4/H, HIST1H4B, HIST1H4I, H4FN, HIST1H4C, H4FI, H4FC, H4/C, H4/E, Histone H4, H4/G, H4/O, H4FB, H4FK, H4/M, H4/B, H4C1, HIST2H4, H4F2, HIST2H4B, HIST1H4A, HIST1H4J, H4/N, H4FH, H4/I, H4/J, H4/K, H4FJ, HIST1H4H, HIST1H4K, HIST4H4, H4FM, H4FA

Purification

Purified by antigen affinity column.

Accession

P62805

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Histone H4 (H4C1) is a ~11 kDa protein. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-H4C1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: 293T cell lysate
Lane 5: HL-60 cell lysate
Lane 6: Raji cell lysate