Anti-Human HACE1 IP Recombinant Antibody (SAA0202) (HP555013)

Name: Anti-Human HACE1 IP Recombinant Antibody (SAA0202)
Brand: abinScience
SKU: HP555013
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP555013

Species reactivity

Human

Applications

ELISA, IF, IP

Host species

Mouse

Isotype

IgG1

Clone ID

SAA0202

Clonality

Monoclonal

Target

HECT-type E3 ubiquitin transferase HACE1, HACE1, E3 ubiquitin-protein ligase HACE1, HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1, KIAA1320

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q8IYU2

RRID

Anti-Human HACE1 IP Antibody (SAA0202) (abinScience Cat# HP555013, RRID:AB_3729736)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

E3 ubiquitin-protein ligase HACE1 is a ~102 kDa protein. E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically binds GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.

1. Anglesio, MS. et al. (2004) Human molecular genetics 13, 2061-74. PMID: 15254018

2. Tang, D. et al. (2011) Nature communications 2, 501. PMID: 21988917

3. Torrino, S. et al. (2011) Developmental cell 21, 959-65. PMID: 22036506

4. Singh, S. et al. (2023) Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10, e2207672. PMID: 37537642

5. Düring, J. et al. (2024) Nature structural & molecular biology 31, 364-377. PMID: 38332367

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human HACE1 IP Antibody (SAA0202)

References

Datasheet

Anti-Human HACE1 IP Recombinant Antibody (SAA0202).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human HACE1 IP Recombinant Antibody (SAA0202) (HP555013)

Overview

Images

References

Datasheet

SDS

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