Anti-Human HINT1 Polyclonal Antibody (HW557014)

Name: Anti-Human HINT1 Polyclonal Antibody
Brand: abinScience
SKU: HW557014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HW557014

Description

Anti-Human HINT1 Polyclonal Antibody (HW557014) is a rabbit polyclonal antibody detecting HINT1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Pongo abelii, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human HINT1 (Met1-Gly126).

Target

Histidine triad nucleotide-binding protein 1, HINT, Adenosine 5'-monophosphoramidase, HINT1, PKCI-1, PRKCNH1, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1, PKCI1

Purification

Purified by antigen affinity column.

Accession

P49773

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Adenosine 5'-monophosphoramidase HINT1 is a ~13 kDa protein. Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide.

1. Chou, TF. et al. (2005) The Journal of biological chemistry 280, 15356-61. PMID: 15703176

2. Weiske, J. et al. (2006) The Journal of biological chemistry 281, 27356-66. PMID: 16835243

3. Chou, TF. et al. (2007) Molecular pharmaceutics 4, 208-17. PMID: 17217311

4. Chou, TF. et al. (2007) The Journal of biological chemistry 282, 15137-47. PMID: 17337452

5. Wang, J. et al. (2012) The journal of physical chemistry. B 116, 6798-805. PMID: 22329685

6. Zhou, X. et al. (2013) Biochemistry 52, 3588-600. PMID: 23614568

7. Shah, R. et al. (2017) Biochemistry 56, 3559-3570. PMID: 28691797

8. Shah, RM. et al. (2018) Journal of molecular biology 430, 2709-2721. PMID: 29787766

9. Strom, A. et al. (2020) FEBS letters 594, 1497-1505. PMID: 31990367

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-HINT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Raji cell lysate
Lane 2: K562 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Hela cell lysate
Lane 5: HepG2 cell lysate
Lane 6: MCF-7 cell lysate

References

Datasheet

Anti-Human HINT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human HINT1 Polyclonal Antibody (HW557014)

Overview

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References

Datasheet

SDS

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