Anti-Human HLA-F Polyclonal Antibody (HM831014)

Name: Anti-Human HLA-F Polyclonal Antibody
Brand: abinScience
SKU: HM831014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HM831014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HLA-F (Gly22-Pro304).

Target

Leukocyte antigen F, CDA12, MHC class I antigen F, HLA F antigen, HLA-F, HLAF, HLA class I histocompatibility antigen, alpha chain F, HLA-5.4

Purification

Purified by antigen affinity column.

Accession

P30511

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

HLA class I histocompatibility antigen, alpha chain F (HLA-F) is a ~39 kDa protein. Non-classical major histocompatibility class Ib molecule postulated to play a role in immune surveillance, immune tolerance and inflammation. Functions in two forms, as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M) and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC). In complex with B2M, presents non-canonical self-peptides carrying post-translational modifications, particularly phosphorylated self-peptides. Peptide-bound HLA-F-B2M acts as a ligand for LILRB1 inhibitory receptor, a major player in maternal-fetal tolerance. Peptide-free OC acts as a ligand for KIR3DS1 and KIR3DL2 receptors.

1. Dulberger, CL. et al. (2017) Immunity 46, 1018-1029.e7. PMID: 28636952

2. Garcia-Beltran, WF. et al. (2016) Nature immunology 17, 1067-74. PMID: 27455421

3. Goodridge, JP. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 3553-62. PMID: 24018270

4. Goodridge, JP. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 1567-77. PMID: 23851683

5. Song, S. et al. (2016) Nature medicine 22, 397-403. PMID: 26928464

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human HLA-F polyclonal antibody at 1ug/mL on recombinant Human HLA-F (Catalog No. HM831012).

References

Datasheet

Anti-Human HLA-F Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human HLA-F Polyclonal Antibody (HM831014)

Overview

Images

References

Datasheet

SDS

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