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Anti-Human HNRNPC Polyclonal Antibody (HY366014)

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Overview
Catalog No.HY366014
Description
Anti-Human HNRNPC Polyclonal Antibody (HY366014) is a rabbit polyclonal antibody detecting HNRNPC in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human HNRNPC (Ser16-Ala85).
Target Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1/C2, HNRNPC, HNRPC
Purification Purified by antigen affinity column.
Accession P07910
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC) is a ~33 kDa protein. Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing.

1. Huang, M. et al. (1994) Molecular and cellular biology 14, 518-33. PMID: 8264621
2. Kim, JH. et al. (2003) Molecular and cellular biology 23, 708-20. PMID: 12509468
3. Shetty, S. (2005) Molecular and cellular biochemistry 272, 107-18. PMID: 16010978
4. Sébillon, P. et al. (1995) Nucleic acids research 23, 3419-25. PMID: 7567451
5. Liu, N. et al. (2015) Nature 518, 560-4. PMID: 25719671
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human HNRNPC Polyclonal Antibody

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