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Anti-Human HSDL2 Polyclonal Antibody (HP427014)

Anti-Human HSDL2 Polyclonal Antibody
Anti-Human HSDL2 Polyclonal Antibody
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Overview
Catalog No.HP427014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human HSDL2 (Met1-Ala290).
Target Hydroxysteroid dehydrogenase-like protein 2, C9orf99, SDR13C1, Short chain dehydrogenase/reductase family 13C member 1, HSDL2
Purification Purified by antigen affinity column.
Accession Q6YN16
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Hydroxysteroid dehydrogenase-like protein 2 (HSDL2) is a ~45 kDa protein. Has apparently no steroid dehydrogenase activity. Controls bile acid (BA) and lipid metabolism in response to nutritional cues.

1. Kowalik, D. et al. (2009) The Journal of steroid biochemistry and molecular biology 117, 117-24. PMID: 19703561
2. Samson, N. et al. (2024) Science advances 10, eadk9681. PMID: 38820148
Note For research use only.
Images
  • Anti-Human HSDL2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-HSDL2 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: 293T cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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