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Anti-Human HSPB11 Polyclonal Antibody (HA518014)

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Anti-Human HSPB11 Polyclonal Antibody
Anti-Human HSPB11 Polyclonal Antibody
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Overview
Catalog No.HA518014
Description
Anti-Human HSPB11 Polyclonal Antibody (HA518014) is a rabbit polyclonal antibody detecting HSPB11 in ELISA, IHC, WB. Suitable for Mus musculus and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human HSPB11 (Met1-Ser144).
Target HSPB11
Purification Purified by antigen affinity column.
Accession Q9Y547
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesIntraflagellar transport protein 25 homolog, Heat shock protein beta-11, Hspb11, Placental protein 25, PP25, HSPB11, C1orf41, IFT25, HSPC034
Background

Intraflagellar transport protein 25 homolog (HSPB11) is a ~16 kDa protein. Component of the IFT complex B required for sonic hedgehog/SHH signaling. May mediate transport of SHH components: required for the export of SMO and PTCH1 receptors out of the cilium and the accumulation of GLI2 at the ciliary tip in response to activation of the SHH pathway, suggesting it is involved in the dynamic transport of SHH signaling molecules within the cilium. Not required for ciliary assembly. Its role in intraflagellar transport is mainly seen in tissues rich in ciliated cells such as kidney and testis. Essential for male fertility, spermiogenesis and sperm flagella formation.

Note For research use only.
Images
  • Anti-Human HSPB11 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-HSPB11 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: A549 cell lysate
    Lane 3: Lncap cell lysate
    Lane 5: K562 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human HSPB11 Polyclonal Antibody

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