Anti-Human HTR2A Polyclonal Antibody (HB201014)

Name: Anti-Human HTR2A Polyclonal Antibody
Brand: abinScience
SKU: HB201014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB201014

Description

Anti-Human HTR2A Polyclonal Antibody (HB201014) is a rabbit polyclonal antibody detecting HTR2A in ELISA, IHC, WB. Suitable for Rattus norvegicus, Pongo pygmaeus, Macaca mulatta, Mus musculus, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HTR2A (Asp2-Asn75&Phe 255-Val324).

Target

HTR2A

Purification

Purified by antigen affinity column.

Accession

P28223

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, Serotonin receptor 2A, HTR2A, HTR2

Background

5-hydroxytryptamine receptor 2A (HTR2A) is a ~52 kDa protein. G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR2A is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways.

1. Stam, NJ. et al. (1992) European journal of pharmacology 227, 153-62. PMID: 1330647

2. Cussac, D. et al. (2008) European journal of pharmacology 594, 32-8. PMID: 18703043

3. Knauer, CS. et al. (2009) Naunyn-Schmiedeberg's archives of pharmacology 379, 461-71. PMID: 19057895

4. Albizu, L. et al. (2011) Neuropharmacology 61, 770-7. PMID: 21645528

5. Delille, HK. et al. (2012) Neuropharmacology 62, 2184-91. PMID: 22300836

6. Cao, D. et al. (2022) Science (New York, N.Y.) 375, 403-411. PMID: 35084960

7. Chen, Z. et al. (2024) Cell 187, 2194-2208.e22. PMID: 38552625

8. Wacker, D. et al. (2017) Cell 168, 377-389.e12. PMID: 28129538

9. González-Maeso, J. et al. (2008) Nature 452, 93-7. PMID: 18297054

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-HTR2A polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse cerebellum lysate
Lane 3: Rat brain lysate
Lane 4: Rat cerebellum lysate