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Anti-Human IDH3A Polyclonal Antibody (HD511014)

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Anti-Human IDH3A Polyclonal Antibody
Anti-Human IDH3A Polyclonal Antibody
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Overview
Catalog No.HD511014
Description
Anti-Human IDH3A Polyclonal Antibody (HD511014) is a rabbit polyclonal antibody detecting IDH3A. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human IDH3A (Gln32-Asp366).
Target EC:1.1.1.41, IDH3A, Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, Isocitric dehydrogenase subunit alpha, NAD(+)-specific ICDH subunit alpha
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P50213
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (IDH3A) is a ~39 kDa protein. Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.

Note For research use only
Images
  • Anti-Human IDH3A Polyclonal Antibody

    Western blot

    Lane 1: Mouse brain lysate
    Lane 2: Mouse heart lysate
    Lane 3: Mouse kidney lysate
    Lane 4: Rat brain lysate
    Lane 5: Rat heart lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human IDH3A Polyclonal Antibody

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